Novel Immortal Cell Lines
Background
Unlike spontaneously immortalized tumor cell lines, which develop from rare late stage, high grade tumors, the vast majority of primary tumors of several organ systems, such as breast, prostate, colon, etc. exhibit a finite mitotic life in cell culture. Although it is widely appreciated that experimentation with in vitro model systems derived from patient tissue could yield clinically relevant surrogate measures for patient management, the limited availability of tumor cells, which proliferate continuously in laboratory dishes, is a major obstacle in this regard.
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Applications
Our lab has designed and adopted multiple approaches for the short term propagation of well-defined tumor cultures from primary breast tumor tissue encompassing the breadth of histologic and clinical heterogeneity. More recently, we have successfully induced cell immortalization by relatively physiological means in tumor epithelium derived from all stages and grades of primary breast cancer. Additionally, the accrual of matched non-malignant patient tissue could provide important tools for cancer risk determination and prevention.
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Advantages
Continuously proliferating clinical samples of tumor and matched non-malignant cells for cancer research.
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Description
A well-established breast cancer cell biologist at the Research Institute at CPMC has made seventeen inventions involving immortalized human breast cancer cells from patient derived primary tumor tissue.
CCd022TT Grade I, stage 1 invasive ductal carcinoma, ER neg/PR neg. Microarray gene expression profiled. hTERT-transduced only.
CCd047TT Grade II, stage 2B invasive ductal carcinoma, ER+/PR+.
Microarray gene expression profiled. hTERT-transduced only.
CCd054TT Grade III, node negative, stage 4 invasive ductal carcinoma. Generates xenografts in SCID/ beige mice. Microarray gene expression profiled. Spontaneously developed, and hTERT-transduced.
CCd061TT Grade II, stage 3 invasive lobular carcinoma, ER+/PR neg.
Matched non-malignant epithelium, and fibroblast cultures. Microarray gene expression profiled. hTERT-transduced only.
CCd066TT Grade II, stage 2 invasive ductal carcinoma, ER+/PR+.
Matched fibroblast cultures. Microarray gene expression profiled. hTERT-transduced only.
CCd067TT Grade I, node-negative, stage 2 invasive ductal carcinoma, ER+/PR+. Matched fibroblast cultures. Microarray gene expression profiled. hTERT-transduced only.
CCd068TT Grade I, stage 2 invasive ductal carcinoma, ER+/PR+, HER2+. Matched fibroblast cultures. Microarray gene expression profiled. hTERT-transduced only.
CCd078TT Grade II, node-negative, stage 1 invasive ductal carcinoma. Matched non-malignant epithelium. hTERT-transduced only.
CCd631TT Grade II, stage 3 invasive mixed ductal lobular carcinoma, ER+/PR neg. Matched fibroblast cultures. hTERT-transduced only.
CCd672TT Grade III, stage 2 invasive ductal carcinoma, ER neg/PR neg.
HER2+. Generates xenografts in SCID/ beige mice. Microarray gene expression profiled. Spontaneously developed, and hTERT-transduced.
CCd675TT Grade III, local recurrence of invasive ductal carcinoma, ER neg/PR+. Spontaneously developed, and hTERT-transduced.
CCd257T Grade III, stage 2B invasive ductal carcinoma, ER neg/PR ±. HER2+. Matched fibroblast cultures. Generates xenografts in SCID/ beige mice. Microarray gene expression profiled. Spontaneously developed only.
CCd329TT Grade II, stage 2B invasive lobular carcinoma, ER neg/PR ±.
Matched fibroblast cultures. hTERT-transduced only.
CCd1570TT Grade II, stage 2A invasive mixed ductal lobular carcinoma, ER+/PR+. Microarray gene expression profiled. hTERT-transduced only.
CCd1599TT Grade II, stage 1 invasive ductal carcinoma. Matched fibroblast cultures. Microarray gene expression profiled. hTERT-transduced only.
CCd1797TT Grade DCIS. hTERT-transduced only. To our knowledge, this is the first human breast epithelial cell line derived from pre invasive disease.
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Patents and Publications
Technology Reference Number
D-011199
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> Novel Anticancer Drugs
> Therapeutics for Invasive and Metastatic Cancers
> ClearVisionTM
> Tracheobronchial Gland Epithelial Cell Lines
> Bronchial Epithelial Cell Lines
> Novel Immortal Cell Lines
> Development of Human Cancer Vaccines in Pre-Clinical Mouse Tumor Models
> Cell Lines For The Study of Respiratory Diseases
> Breast Cancer Risk Assessment through Mammographic Density Measurements