Basic Science Researcher
John Muschler, PhD
Examining cellular responses to the extracellular matrix that underlie cancer progression
The Muschler lab is investigating how alterations in cancer cell surfaces, especially in the extracellular matrix (ECM) receptor dystroglycan, arise and contribute to a wide range of cancers.
Restoring dystroglycan function in a tumor cell line reduces cancer potential. In breast cancer cells, dystroglycan anchors laminin—a protein critical to ECM assembly—to the cell surface. The loss of laminin alters both the ECM and cell interactions with the matrix, and may contribute to metastasis.
Dr. Muschler completed his Ph.D. at the University of Illinois, and he was a post-doctoral fellow at both the Pasteur Institute and the Lawrence Berkeley National Laboratory.
Publications by John Muschler, PhD Opens new window in PubMed.
Representative publications include:
- Leonoudakis, D., Singh, M., Mohajer, R., Mohajer, P., Fata, J.E., Campbell, K.P., Muschler, J.L. (2010, in press). Dystroglycan controls signaling of multiple hormones through modulation of STAT5 activity. J. Cell Science.
- Muschler, J.L., Streuli, C.H. (2010, in press). Cell-matrix interactions in mammary gland development and breast cancer. In Mammary Gland Biology. Cold Spring Harb Perspect Biol doi: 10.1101/cshperspect.a003202 (peer-reviewed book chapter)
- Xu, R., Nelson, C.M., Muschler, J.L., Veiseh, M., Vonderhaar, B.K., Bissell, M.J. (2009). Sustained activation of STAT5 is essential for chromatin remodeling and maintenance of mammary-specific function. J. Cell Biology. 184:57-66.
- Oppizzi, M.L., Akhavan, A., Singh, M., Fata, J.E., Muschler, J.L. (2008). Nuclear Translocation of Beta-Dystroglycan Reveals a Distinctive Trafficking Pattern of Autoproteolyzed Mucins. Traffic. 9:2063-2072
- Akhavan, A., Crivelli, S., Singh, M., Lingappa, V.R., Muschler, J.L. (2008). SEA domain proteolysis determines the functional composition of dystroglycan. The FASEB Journal. 22:612-21 (published online September, 2007)
- Weir, L., Oppizzi, M.L., Henry, M.D., Onishi, A., Campbell, KP., Bissell, M.J., Muschler, J. (2006) Dystroglycan loss disrupts polarity and beta-casein induction in mammary epithelial cells by perturbing laminin anchoring. J. Cell Science. 119:4047-58.
- Singh, J, Itahana, Y, Knight-Krajewski, S., Kanagawa, M., Campbell, KP., Bissell, M.J., Muschler J. (2004) Proteolytic enzymes and altered glycosylation modulate dystroglycan function in carcinoma cells. Cancer Research. 64: 6152-9.
- Kanagawa, M., Saito, F., Kunz, S., Yoshida-Moriguchi, T., Barresi, R., Kobayashi, Y. M., Muschler J., Dumanski, J. P. , Michele, D. E., Oldstone, M. B., and Campbell, K.P. (2004) Molecular recognition by LARGE is essential for expression of functional dystroglycan. Cell. 117: 953-64
- Weir, M.L. and Muschler J. (2003) Dystroglycan: emerging roles in mammary gland function. J. Mammary Gland Biol. Neoplasia 8 (4): 409- 420.
- Muschler J., Levy, D., Boudreau, R., Henry, M. and Campbell, K.C. and Bissell, M.J. (2002). A role for dystroglycan in epithelial polarization: loss of function in breast tumor cells. Cancer Research. 60: 7102-09.
- Radisky, D., Muschler J. and Bissell, M.J. (2002). Order and disorder: the role of extracellular matrix in epithelial cancer. Cancer Investigations. 20 (1): 1-16.
Visit www.cpmcri-currents.org for the latest news about Dr. Muschler's research.
John Muschler, PhD., Armin Akhavan, Ph.D., Pouya Mohajer, M.D., Dmitri Leonoudakis, Ph.D., Jennifer Ng (UC Berkeley student), and Manisha Singh, M.S.