Giuseppe Inesi, MD, PhD
Email: InesiG@cpmcri.org
Research Areas
1) Mechanisms of free energy transduction in enzymes
2) Active transport (Ca2+) in biomembranes
3) Excitation-contraction coupling in skeletal and cardiac muscle
4) Ca2+ and signal transduction
5) Gene transfer in Eukaryotic cells
Dr Inesi's laboratory has been engaged in a long term research project on Molecular and Cellular Aspects of the Ca2+ Transport ATPase. This enzyme is a membrane bound protein that utilizes ATP for active transport of Ca2+, and is required for storage of Ca2+ in intracellular stores. In turn, release of the stored Ca2+ is required for intracellular signaling and initiation of most cellular functions as a second messenger. Dr Inesi's molecular studies include determination of enzyme and transport kinetics, protein chemistry and derivatization of various amino acid residues, and production of recombinant protein to obtain specific mutations and assess their effects on catalytic and transport function. Cellular studies involve overexpression or silencing of the ATPase gene in cultured cells, and determination of functional consequences with regard to Ca2+ signaling and regulation of Ca2+ dependent functions. This research is currently supported by a five year National Institutes of Health (NIHLBI) award.
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Figures
Education
1954 University of Modena, Italy Doctor in Medicine
1960 University of Bologna, Italy Docence (Ph.D)
1958-62 University of Pennsylvania, Post doctoral Fellow
1963-65 University of California San Francisco, Postdoctoral Fellow
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Academic Appointments
1965-70 Established Investigator of the American Heart Association
1965-69 University of California Assistant Professor
San Francisco, California Cardiovascular Research Institute
1969-72 Carnegie-Mellon University Tenured Associate Professor
Pittsburgh, Pennsylvania (Biochemistry)
1972-81 University of the Pacific Tenured Professor
San Francisco, California (Physiology)
1981-2006 University of Maryland Tenured Professor
Biochemistry and Mol Biol
Chairman (1983-2006)
Baltimore, Maryland
1989-90 Stanford University Visiting Professor
Stanford, California
1996-97 University of California Visiting Professor
San Francisco, California
2005 University of Florence, Italy
Visiting Professor
2006 Calif. Pacific Med. Center Res. Inst.
Senior Scientist
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Publications
Click here for List of publications by Giuseppe Inesi, MD, PhD in PubMed.
Selected articles:
Clarke DM, Loo TW, Inesi G, MacLennan DH Location of high affinity Ca2+-binding sites within the predicted transmembrane domain of the sarcoplasmic reticulum Ca2+-ATPase. Nature. 1989;339: 476-8
Inesi G Mechanism of Calcium Transport. Annual Review of Physiology 47:573-601 1985
Inesi G, Kurzmack M., Coan C, Lewis DE. Cooperative calcium binding and ATPase activation in sarcoplasmic reticulum vesicles. J Biol Chem. 1980; 255: 3025-31
Sagara Y, Inesi G Inhibition of the sarcoplasmic reticulum Ca2+ transport ATPase by thapsigargin at subnanomolar concentrations. J Biol Chem. 1991; 266:13503-6.
Scarpa A, Baldassare J, Inesi G The effect of calcium ionophores on fragmented sarcoplasmic reticulum. J Gen Physiol. 1972; 60: 735-49.
Chiesi M, Inesi G Adenosine 5'-triphosphate dependent fluxes of manganese and and hydrogen ions in sarcoplasmic reticulum vesicles. Biochemistry. 1980;19: 2912-8.
Inesi G, Lewis D, Toyoshima C, Hirata A, de Meis L. Conformational fluctuations of the Ca2+-ATPase in the native membrane environment. Effects of pH, temperature, catalytic substrates, and thapsigargin. J Biol Chem. 2008; 283:1189-96.
Prasad AM, Ma H, Sumbilla C, Lee DI, Klein MG, Inesi G Phenylephrine hypertrophy, Ca2+-ATPase (SERCA2), and Ca2+ signaling in neonatal rat cardiac myocytes. Am J Physiol Cell Physiol. 2007; 292: C2269-75.
Inesi G, Lewis D, Ma H, Prasad A, Toyoshima C. Concerted conformational effects of Ca2+ and ATP are required for activation of sequential reactions in the Ca2+ ATPase (SERCA) catalytic cycle. Biochemistry. 2006; 45:13769-78.
Toyoshima C, Inesi G Structural basis of ion pumping by Ca2+-ATPase of the sarcoplasmic reticulum. Annu Rev Biochem. 2004;73:269-92.
Tadini-Buoninsegni F, Bartolommei G, Moncelli MR, Guidelli R, Inesi G Pre-steady state electrogenic events of Ca2+/H+ exchange and transport by the Ca2+-ATPase. J Biol Chem. 2006; 281: 37720-7
Prasad AM, Inesi G Effects of Thapsigargin and Phenylephrine on Calcineurin and Protein Kinase C Signaling Functions in Cardiac Myocytes. Am J Physiol Cell Physiol. 2009 Feb 25.
WEB OF SCIENCE lists 11.807 citations of Dr Inesi’s articles as of March 2009, with h-index = 61.
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