Dieter C. Gruenert, PhD
Email: GrueneDC@cpmcri.org
Dieter Gruenert's Lab:
Jahan Marcu
Email: marcuj@cpmcri.org
Hamid Emamekhoo, MD
Email: emamekh@cpmcri.org
Hooman Parsi, MD
Email: Parsih@cpmcri.org
Biography
Dieter Gruenert's Bio PDF format
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Research Projects
A primary thrust of the research has been to understand the mechanism(s) underlying homologous replacement in human somatic cells with the ultimate aim of developing cellular and genetic therapeutic strategies for cystic fibrosis (CF) and sickle cell disease (SCD) as well as other monogenic and multigenic diseases. We have been engaged in studies to develop a novel approach to gene targeting and have defined a new paradigm for viewing homologous replacement in human cells. In addition, we are investigating the therapeutic potential of pluripotent and totipotent human stem cells. This work has coupled with investigations into the development of transgenic large animal models of disease using nuclear transfer and cloning technologies. In that context, we are currently investigating the possibility of developing a transgenic pig for cystic fibrosis.
In addition to the gene targeting and stem cell biology studies, we are investigating the role of connexins in the bystander killing phenomenon observed in the suicide gene killing of tumor cells. We have initiated studies in both human airway epithelial derived cells and in brain tumor derived cells. As an outgrowth of this work, we are analyzing the connexin gene profile of the both transformed and non-transformed cells in brain and in the airway epithelium. These studies are aimed at defining the role that intercellular communication plays in the modulation of cell proliferation and in neoplastic progression.
In the context of the Human Genome Project, ongoing studies to isolate full-length cDNAs, that are candidate genes for breast cancer are underway. We have developed a novel method for generating full-length cDNAs in a rapid and cost-efficient fashion that should expedite the program in gene discovery. In conjunct with this program, we are engaged in studies directed at elucidating the novel genetic mechanisms underlying neoplastic progression and cell transformation.
We also have a continuing program in the generation and characterization of immortalized cell lines for human genetic disorders. In part, these cells lines have been used as tools to investigate the biological pathways associated with various pathologies that accompany inherited diseases. Employing high throughput screening strategies, these cells have also been used to develop strategies for modifying the biochemical pathways that lead to a given pathology and have led to a better understanding of the functional significance of specific genes. These cell lines have been used by numerous laboratories, both nationally and internationally, to elucidate the cellular and genetic mechanisms that underlie human airway epithelial disease, genetic disorders such as cystic fibrosis, xeroderma pigmentosum, fragile X syndrome, and airway epithelial cell and cancer biology.
As a component of our stem cell program, studies have been initiated to isolate and purify hematopoietic stem cells for ex vivo gene therapy of hemaglobinopathies and other inherited diseases treatable by autologous stem cell transplantation as well as defining growth conditions that will allow lineage specific differentiation of pluripotent and totipotent stem cells. The roles of specific cytokines and tissue environments in the differentiation of these stem cells are under investigation in vitro and in vivo.
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Epithelial Cell Lines
Epithelial Cell Lines For The Study of Respiratory Diseases
We have been engaged in the isolation, separation and further development of cell lines and creation of cellular systems for clinical and research use. We have a library of bronchial, nasal and airway epithelial cell lines from human, rabbit, pig and mice. These cells represent the spectrum of cells affected in respiratory diseases and can be used to research and develop new drugs for Cystic Fibrosis, Asthma, lung cancer, and Avian Flu.
Links
NIH National Human Genome Research Institute
NIH Grants & Funding Opportunities
American Society of Gene Therapy
Cystic Fibrosis Worldwide
Cystic Fibrosis Foundation
Pennsylvania Cystic Fibrosis, Inc.
European Cystic Fibrosis Society
American Cancer Society
American Heart Association
American Lung Association
Juvenile Diabetes Foundation
American Society of Hematology
California Institute of Regenerative Medicine
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Cell Culture
Cell Lines Used in CF Research
Cell Protocols
Cell Line Reference
Frequently Asked Questions
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Publications
Click here for List of publications by Dieter C. Gruenert, PhD in PubMed.
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