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    Cells Viewed by Time-lapse Microscopy Video

    This video is for our Study Reviewers only. Questions about this video can be directed to Garret L. Yount, PhD at YountG@cpmcri.org

    The time-lapse video highlights the spontaneous formation of independent motile microplasts (IMMPs) from human glioblastoma cells migrating within a 3-D complex that mimics tissue structure. As seen under standard culture conditions, lamellar protrusions are able to break away from glioblastoma cell bodies and immediately reorganize into smaller, organized mobile units. The video follows one cell for 72 hours (filled arrow at start of video) as it undergoes mitosis and one of the daughter cells extends lamellar protrusions that break away as IMMPs (blue circles). The IMMPs display the morphological characteristics typical of cell locomotion, including establishment of polarity, extension of lamellipodia, retraction of the trailing edge, changing direction of movement, and the ability to maintain integrity as an intact unit when encountering a neighboring IMMP.